https:\/\/doi.org\/10.1007\/s41981-024-00329-w<\/a><\/p>\nAbstract<\/p>\n
The separation of silybin A (SilA<\/b>) and B (SilB<\/b>) diastereomers in optically pure compounds is challenging due to their very similar physical and chemical properties. However, such separation is crucial for evaluating the biological activity of the diasteroisomers\u00a0SilA<\/b>\u00a0and\u00a0SilB<\/b>, which show very different performance in pharmacological applications like treating prostate cancer, liver diseases, and Alzheimer\u2019s disease. The most common isolation method is based on high-performance liquid chromatography, but it is slow and has a yield in pure\u00a0SilB<\/b>\u00a0of hundreds of milligrams per day. An alternative chemo-enzymatic separation method, utilizing an immobilized lipase CALB catalyst to stereoselectively acetylate silybin B (1b<\/b>), offers advantages in terms of higher productivity, selectivity, and scalability, particularly when applied in flow reactors. This study delves into the kinetics of\u00a0Sil<\/b>\u00a0acetylation catalyzed by Novozym 435 in a continuous flow milli-reactor, investigated at various temperatures, volumetric flow rates, and Sil initial concentrations. It is noteworthy that, at the current state of the art, there is a lack of kinetic studies on this reaction, emphasizing the novelty and significance of this work. The kinetic and fluid dynamic parameters were estimated using a non-linear regression analysis of experimental data. The examined reaction showed a null apparent activation energy, explaining the temperature insensitivity of the final acetylated silybin B (1b<\/b>) concentration. Furthermore, the decrease in steady-state concentrations of the acetylated products with increasing volumetric flow rates indicated that the reaction was occurring in a kinetic regime. Interestingly, a maximum starting Sil concentration was identified, above which there was no favorable impact on conversion.<\/p>\n","protected":false},"excerpt":{"rendered":"
Novel insights into acetylation kinetics in a continuous Flow milli-reactor for chemo-enzymatic separation of silybin A\/B Michele Emanuele Fortunato, Rita Pagano, Valeria Romanucci, Chiara Licenziato, Armando Zarrelli, Martino Di Serio, Giovanni Di Fabio, Vincenzo Russo Journal of Flow Chemistry, 14, 585\u2013595 https:\/\/doi.org\/10.1007\/s41981-024-00329-w Abstract The separation of silybin A (SilA) and B (SilB) diastereomers in optically […]<\/p>\n","protected":false},"author":2,"featured_media":2219,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4,17],"tags":[],"class_list":["post-2217","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-homepage","category-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/posts\/2217","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/comments?post=2217"}],"version-history":[{"count":1,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/posts\/2217\/revisions"}],"predecessor-version":[{"id":2220,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/posts\/2217\/revisions\/2220"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/media\/2219"}],"wp:attachment":[{"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/media?parent=2217"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/categories?post=2217"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nicl.it\/new\/wp-json\/wp\/v2\/tags?post=2217"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}